Last updated March 2022

Anti-wrinkle injections are the most popular cosmetic procedure in the world today. It is a simple yet effective procedure; a few injections, no downtime, and wrinkles disappear. Despite this, to produce natural and consistent results requires experience and an understanding of the underlying facial anatomy. Anti-wrinkle injections have been performed at the Victorian Cosmetic Institute’s Melbourne clinics since 2005. During this time, we have constantly refined our methods to produce the best outcomes and experiences for our patients.

What are anti-wrinkle injections?

Anti-wrinkle injections aid in wrinkle reduction formed by the muscles of facial expression. Composed of a commercially prepared form of Clostridium botulinum, this causes a block in the nerve activity to the injected facial muscles, providing a temporary reduction in muscle movement.

How do anti-wrinkle injections work?

Anti-wrinkle injections are a commercially prepared from of Clostridium botulinum toxin. When injected, it is possible to target and relax specific facial muscles and reduce facial wrinkling. When the muscles of facial expression contract, they may crease the skin and cause wrinkles. Continual contraction unabated over time may result in expression lines, however anti-wrinkle injections may help to prevent this.
The effect is temporary, lasting roughly 3-4 months with the treatment requiring repetition to maintain the effect.
The frown, forehead, and around the eyes are commonly treated areas. The treatment aims to relax facial muscles and expression, and by adjusting the dynamics and interplay between facial muscles, it is possible to improve the aesthetics of the face.


  • Anti-wrinkle injections help to relax certain wrinkle-causing muscles in the face to soften fine lines and produce a younger and more refreshed appearance.
  • Injectables are relatively painless when performed using special techniques including ice, vibration and the injecting gun.
  • Using tools such as the Accuvein Vein Finder and injecting guns, bruising and discomfort can be reduced.
  • The injectables can be used to treat a variety of non-surgical procedures, including teeth grinding and excessive sweating.
  • The treatment has a proven safety record for over 25 years.
  • Our team of Cosmetic Doctors and Cosmetic Nurses have extensive experience in injectables and abide by safety standards and protocols to achieve optimum results.

What else can be treated with anti-wrinkle injections?

Anti-wrinkle injections can also be used for a variety of medical conditions. These include; teeth grinding or clenching, cervical dystonia, facial spasms, spasticity and migraine prevention.

When injected into the skin of the armpits, hands, or feet, anti-wrinkle injections can reduce excessive sweating in these areas for approximately 8 months.

What are the most common facial areas treated?

Anti-wrinkle injections are most commonly used for the treatment of frown lines, forehead lines, and crows feet or smile lines.

However, they can also be used to;

Certain muscles around the eyes are relaxed to help create an overall lift in the eyebrows.

Helps reduce the pull of muscles on the mouth and lower face.

The injections are used to lower the upper lip. Dermal filler can then be used to thicken the upper lip.

  • evert the upper lip (also known as the lip flip)

Relaxes the muscles to reduce the amount of pursing that causes wrinkling on the upper lip.

Minimises the drag of the muscle on the face, improves the jawline, and reduces the prominence of neck bands.

  • reduce bunny lines on the nose

Lessens the appearance of bunny lines on the nose by relaxing the muscles.

  • reduce dimpling of the chin

Anti-wrinkle injections can be injected to help relax the chin to prevent the appearance of orange peel like dimpling.

Injections are used in the main muscle that causes the bulk, and reduces bruxism and squareness of the jaw.  This can help slim the jawline and relieve associated headaches or pain.

In addition to this, anti-wrinkle injections can also be used to treat armpit, hand, feet and facial sweating.

Case Studies

Jawline slimming

This patient presented to the Victorian Cosmetic Institute for jawline slimming. She had anti-wrinkle injections in each of her masseter muscles, which work as a muscle relaxant reducing the size and firmness of the masseter muscle. This treatment has both aesthetic and functional purpose. Aesthetically, the jawline appears slimmer and more defined. Functionally, relaxing this muscle can help reduce headaches, jaw pain, and dental issues such as teeth grinding and clenching.

jawline slimming with botox before and after

Combining anti-wrinkle injections and filler

This patient presented to Victorian Cosmetic Institute with concerns of the way her frown lines made her appear. She is a happy and joyful person and wanted her face to reflect her kind nature. The result is a youthful and natural appearance with no surgery. The static lines have been smoothed using dermal filler and reduced the cause of her frown lines with anti-wrinkle injections.

Frown and forehead before and after (1)

What does treatment involve?

Photographs of the face and videos of facial expressions are required prior to each treatment and follow-up appointments for before and after treatment comparison. Standardised photography (camera equipment, lighting, camera settings) is used to ensure that the only changes are the changes in the subject.

Careful cleaning of the face with anti-septic to remove make-up and skin pathogens is the first step of treatment.

We then use the Accuvein vein finder to establish the locations of any veins in the treatment areas. Visualising and avoiding veins helps to reduce bruising post-treatment.

The muscle movements of the area(s) to be treated are then assessed, and injection points marked to ensure the accurate placement of injections. These markings may also be photographed and recorded for the purposes of documentation for future treatments.

The anti-wrinkle injections are injected with a fine needle and syringe, using an injecting gun, into the pre-marked areas. The injecting gun allows for a more precise injection than by hand. Depth and dosage are highly accurate using this method. Furthermore, discomfort is significantly reduced as the movement of the needle through the skin is significantly faster and straighter than what can be performed by hand. Ice cubes or a vibration device can also be used on the treatment area prior to injection. This also significantly reduces discomfort.

After treatment, the skin is cleansed again with anti-septic, removing any blood or markings.


Can I resume normal activities after treatment?

We do advise to not massage the treatment areas after or perform vigorous exercise that may shift the positioning of the anti-wrinkle injections. Otherwise, normal daily activities can be continued immediately post-treatment.

When will I see results?

Anti-wrinkle injections generally have an onset action of 4 to 7 days to fully develop, with the average longevity of roughly 3 to 4 months.

How long do anti-wrinkle injections last?

The duration of effectiveness is dependent on the dose administered and the strength of the muscle injected. On average, anti-wrinkle injections last for 3-4 months. Those who have a fast metabolism may require more regular treatment to maintain their results.

Is it possible to become resistant to anti-wrinkle treatments?

It is possible to become immune to some brands of anti-wrinkle injections, as they need to be performed regularly to maintain results.

The active component of the treatment is a bacterial toxin that does not cause an immune response. However, the associated components that are manufactured with the bacterial toxin, including complexing proteins and inactive toxin, may cause an immune response. Therefore, neutralising antibodies are formed that cause future treatments to be partially or completely ineffective, however the associated components have no clinical effect.

To prevent patients becoming resistant to anti-wrinkle injections we only administer botulinum toxin that does not contain the complexing proteins that may cause an immune response.

Why do anti-wrinkle injections stop working?

The greater the dose, and the more frequent the treatment, the more likely the immunity will occur. It is possible that neutralising antibodies have been produced by the body’s immune system and therefore, may act as a vaccine onto itself.

To prevent this, at the Victorian Cosmetic Institute, we utilise a purified version of the anti-wrinkle injections, which has the least risk of causing an immune response and subsequent non-responsiveness to treatment.

However, once immunity has been conferred, it is important to note that none of the brands will work – even the purified version. In the case of immunity, it is necessary to wait, often for years before the immunity is reversed.

Are anti-wrinkle injections safe?

Anti-wrinkle injections have been used for cosmetic purposes for over twenty years and for medical purposes for even longer without any reports of long-term side effects.

What are the side effects of anti-wrinkle injections?

Generally, there are few side effects to treatment, and no downtime involved. Occasionally, a bruise may appear over the treated area, but is more common around the eyes. This can be covered with make-up if applicable. There is usually some transient redness and swelling of the treated area (similar to a mosquito bite), and this usually lasts for less than one hour. Other possible adverse outcomes include; asymmetrical results, failure to cause a significant change in the wrinkles (usually due to inadequate dosing), puffy eyes (for treatments around the eyes), and a drooping mouth corner (from misplacement of the injections).

When treating the frown with anti-wrinkle injections, approximately one percent of people experience movement of the product into one eyelid and this causes a partial eyelid droop that can last for one month. Any side effect of treatment is temporary and will resolve naturally. A particular eyedrop can be given to temporarily reduce the effect of this side effect if necessary.

Anti-wrinkle before and after case studies

The following are examples of anti-wrinkle injections injected into smile lines or crows feet, forehead lines, frown lines, neck or platysmal bands and a gummy smile.

anti-wrinkle case study photo

botox Melbourne

botox Melbourne

botox melbourne

botox melbourne

How much do anti-wrinkle injections cost?

This depends on the muscle strength and desired outcome, and whether the goal is to reduce or stop movement altogether. Each area and person requires a different number of units, so the per-unit pricing is more consistent and fair.

The price we charge allows us to provide a premium service with the best outcome. We have experienced injectors with excellent technical and aesthetic skills, systems and protocols to achieve consistent results and should there be any concerns, a free top-up is provided.

See our anti-wrinkle injection pricing.

Why should I choose Victorian Cosmetic Institute as my provider?

Our dedicated team of cosmetic doctors and nurses have years of extensive experience in performing anti-wrinkle injections.

We allow you ample time to communicate your goals to us and use our expertise to help you achieve your desired outcome. It is paramount that our patients have a good relationship with their practitioner and the experience you have with us is also important. We offer various ways to help alleviate any discomfort or anxiety from the procedure.

Simply contact us for your initial consultation, where we will discuss with you what is a realistic and achievable outcome, and what to expect from your treatment.

It’s as easy as clicking the ‘book online’ button or you can phone us directly on 1300 863 824.